An experiment to look at the effect of 1 factor on an enzyme

Aim To design and carry out an investigate to encounter at one factor which affects the enzyme catalase. Catalase is involved in the crack-up of total heat Peroxide into water and atomic result 8. 2H202 _ 2H20 + 02 It can be found in most cells, nonetheless it is mostly concentrated in the liver, kidney and genus Tuber cells of plants (e.g. potato). Hypothesis I predict that as the temperature increases the atomic number 8 produced swallow up increase until a point (optimum temperature), then leave alone lessen until no oxygen is produced ( due to denaturation ) Justification All enzymes make up optimum conditions, at which they work best. therefrom in my experiment there forget be an optimum temperature i.e. the temperature where oxygen is accustomed off most rapidly. I stated that at a temperature no oxygen would be produced. This is because I cogitate the enzyme result denature due to the high temperature ( ie the tertiary complex body par t allow change ). Due to the denaturation of the enzyme, no oxygen will be produced. I also feel it is important to money box note that the H2O2 is an unstable compound and at high temperatures (100aC) it will disintegrate by itself.

Apparatus For my experiment I will need the chase frame-up: h Conical flask with side on delivery outlet h close-fitting fitting bung for the conic flask h spoken communication tube h Gas-syringe h Water bathroom h Retort stand h Boss and clamp I will set the apparatus up in the following way ¡V Control of variables FactorControl TemperatureI have elect to veer this fact or using an electronic water bath in my expe! riment. ConcentrationI will use the substrate from the same bottleful without dilution. I will take my cuttings of potato from the same region. Surface AreaI will use the same cork borer and cut every(prenominal) of the potato to the... If you want to get a full essay, ordination it on our website: BestEssayCheap.com

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